PRIMER PICKING RESULTS FOR NM_006162_9 Using mispriming library /data/utswmc/elxr/ELXR-main/repbase/humrep_and_simple.fa Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 247 21 59.35 42.86 5.00 3.00 12.00 tcacactcattcgcaacttgt RIGHT PRIMER 734 20 61.15 60.00 4.00 2.00 12.00 cagagcgagtgttcgagacc SEQUENCE SIZE: 983 INCLUDED REGION SIZE: 983 PRODUCT SIZE: 488, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 269,443 0 aaacccacaggcccctgggtccaggactcccactgcagtttgcaagtggacttttatagc 60 acgttagtgctgcaggaggttccaaggaaaacagtcctcgccagtattagaaccagctgc 120 tactctctcggccgatgactcacgccttttttttttaatggggtgggagaaccaggcagg 180 agacagagggacccagtcagggccctgttggttttgcggagggtgccccagccacaagcc 240 tgcctgatcacactcattcgcaacttgtgtttatttaattttttttttccttctcacagT >>>>>>>>>>>>>>>>>>>>> ******************************* 300 TCCAATTATAAAAACAGAACCCACTGATGATTATGAGCCTGCTCCAACCTGTGGACCGGT ************************************************************ 360 GAGCCAGGGGTTAAGTCCTCTCCCAAGACCATACTACAGCCAGCAGCTCGCGATGCCACC ************************************************************ 420 CGACCCCAGCTCCTGCCTCGTGGCCGGCTTCCCGCCCTGTCCGCAGAGAAGCACCCTGAT ************************************************************ 480 GCCAGCGGCCCCTGGCGTGAGCCCCAAGCTCCACGACCTTTCTCCCGCTGCCTACACCAA ************************************************************ 540 GGGCGTTGCCAGCCCGGGCCACTGTCACCTCGGACTCCCGCAGCCGGCCGGAGAGGCCCC ************************************************************ 600 CGCCGTCCAGGACGTGCCCAGGCCAGTGGCCACGCACCCCGGCTCGCCCGGGCAGCCACC ************************************************************ 660 CCCGGCCCTGCTGCCACAGCAGgtgagtgcgcctccaagcagtagctgcccccctggtct **************************************************** <<<<< 720 cgaacactcgctctgccccagcagcccctctcctccactcccgcctgccacccaagagcc <<<<<<<<<<<<<<< 780 gacctgcctgcagccctgcagcccagcgtgcccgcccgccacgggccgcccgcagcacct 840 gccgtccacggtccgcagggacgagtctccgactgccgggccacggctgctgccagaggt 900 gcatgaggacggtagtcctaatttggcccctattcctgtaacggtcaagcgagagcctga 960 agagttggaccagttgtacctgg KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 238 21 60.67 52.38 6.00 0.00 11.00 cctgcctgatcacactcattc RIGHT PRIMER 734 20 61.15 60.00 4.00 2.00 12.00 cagagcgagtgttcgagacc PRODUCT SIZE: 497, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 247 21 59.35 42.86 5.00 3.00 12.00 tcacactcattcgcaacttgt RIGHT PRIMER 735 19 60.44 57.89 4.00 2.00 12.00 gcagagcgagtgttcgaga PRODUCT SIZE: 489, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00 3 LEFT PRIMER 238 21 60.67 52.38 6.00 0.00 11.00 cctgcctgatcacactcattc RIGHT PRIMER 735 19 60.44 57.89 4.00 2.00 12.00 gcagagcgagtgttcgaga PRODUCT SIZE: 498, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00 4 LEFT PRIMER 246 22 59.67 40.91 5.00 3.00 12.00 atcacactcattcgcaacttgt RIGHT PRIMER 734 20 61.15 60.00 4.00 2.00 12.00 cagagcgagtgttcgagacc PRODUCT SIZE: 489, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00 Statistics con too in in no tm tm high high high high sid many tar excl bad GC too too any 3' lib poly end ered Ns get reg GC% clamp low high compl compl sim X stab ok Left 1343 0 0 0 402 0 0 2 14 395 35 22 54 419 Right 1684 0 0 0 1271 0 0 0 0 145 25 0 32 211 Pair Stats: considered 9532, unacceptable product size 9525, ok 7 primer3 release 1.0