PRIMER PICKING RESULTS FOR NM_006162_8 Using mispriming library /data/utswmc/elxr/ELXR-main/repbase/humrep_and_simple.fa Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 103 20 59.74 55.00 3.00 2.00 12.00 gtggctgcttgggagtttac RIGHT PRIMER 563 21 60.02 47.62 2.00 0.00 12.00 tcactaacgggacatcacaca SEQUENCE SIZE: 733 INCLUDED REGION SIZE: 733 PRODUCT SIZE: 461, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00 TARGETS (start, len)*: 269,193 0 gaagccaagggcagccccttccctgtgagcgtgcgtgtccacacgcgtgtgcaggtggca 60 cagagcatccagggctgtcatcagctgcttctggaccgcccaggtggctgcttgggagtt >>>>>>>>>>>>>>>>> 120 taccgtcacggcagtcctgtgctgccgtggaaacgcggggttgccgtgtggccgccgtgg >>> 180 aaacgcggggttgccgtgtggccgccgtggaaacgcggggttgccgtgtggccgccgtgg 240 cgcggcagccatcgcctgcccggtgctgattgtgcctcctgtgtgcccttctcctgtagA ******************************* 300 ATTCTCTGGTGGTTGAGATCCCGCCATTTCGGAATCAGAGGATAACCAGCCCCGTTCACG ************************************************************ 360 TCAGTTTCTACGTCTGCAACGGGAAGAGAAAGCGAAGCCAGTACCAGCGTTTCACCTACC ************************************************************ 420 TTCCCGCCAACGgtaacgccatctttctaaccgtaagccgtgaacatgagcgcgtggggt ****************************************** 480 gctttttctaaagacgcagaaacgacgtcgccgtaaagcagcgtggcgtgttgcacattt 540 aactgtgtgatgtcccgttagtgagaccgagccatcgatgccctgaaaaggaaaggaaaa <<<<<<<<<<<<<<<<<<<<< 600 gggaagcttcggatgcattttccttgatccctgttgggggtggggggcgggggttgcata 660 ctcagatagtcacggttattttgcttcttgcgaatgtataacagccaaggggaaaacatg 720 gctcttctgctcc KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 103 20 59.74 55.00 3.00 2.00 12.00 gtggctgcttgggagtttac RIGHT PRIMER 567 21 59.98 52.38 4.00 1.00 12.00 ggtctcactaacgggacatca PRODUCT SIZE: 465, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00 2 LEFT PRIMER 74 20 59.73 55.00 6.00 3.00 12.00 ctgtcatcagctgcttctgg RIGHT PRIMER 563 21 60.02 47.62 2.00 0.00 12.00 tcactaacgggacatcacaca PRODUCT SIZE: 490, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 3 LEFT PRIMER 74 20 59.73 55.00 6.00 3.00 12.00 ctgtcatcagctgcttctgg RIGHT PRIMER 567 21 59.98 52.38 4.00 1.00 12.00 ggtctcactaacgggacatca PRODUCT SIZE: 494, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 4 LEFT PRIMER 109 20 59.60 55.00 3.00 3.00 11.00 gcttgggagtttaccgtcac RIGHT PRIMER 563 21 60.02 47.62 2.00 0.00 12.00 tcactaacgggacatcacaca PRODUCT SIZE: 455, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 Statistics con too in in no tm tm high high high high sid many tar excl bad GC too too any 3' lib poly end ered Ns get reg GC% clamp low high compl compl sim X stab ok Left 1680 0 0 0 1282 0 0 0 9 113 22 0 34 220 Right 1284 0 0 0 205 0 0 0 6 254 52 0 72 695 Pair Stats: considered 9, unacceptable product size 2, ok 7 primer3 release 1.0