PRIMER PICKING RESULTS FOR NM_006162_7 Using mispriming library /data/utswmc/elxr/ELXR-main/repbase/humrep_and_simple.fa Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 61 19 60.12 57.89 4.00 0.00 11.00 cgtgtcctcagcttctcca RIGHT PRIMER 544 21 59.25 47.62 6.00 2.00 11.00 ctttggcgagtacttggtgat SEQUENCE SIZE: 656 INCLUDED REGION SIZE: 656 PRODUCT SIZE: 484, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 269,116 0 ggatgcccctgccctgtagtccctcctacgccgggctggggtcagaacaagccctgagcc 60 gcgtgtcctcagcttctccaggctcccagggcaactttggtgtcgagctcggggccctgc >>>>>>>>>>>>>>>>>>> 120 cagggcacgtggctcctgaggtgctgccctcgacaaagccctgggatgggcctacgtggg 180 tctcctgggacaaggcgccctcctggctcagggccctgggtctggatcacgtgggggtgt 240 ctggggagggggctccccaccacacagagtcacagacgtttcctgctcctttcttccagA ******************************* 300 TGGCCACCATGTCTGGGAGATGGAAGCGAAAACTGACCGGGACCTGTGCAAGCCGgtgag ************************************************************ 360 tgcctttggcgcagctggagctactgtgggtccccagggcttgggaggctggggtctgct ************************* 420 ggagccactgcgggtccccaggccttgggaggctggggttcctgtttcttagaatgaaac 480 aaataaaaatagtgcatagagtaacagaaccagtaacaaacaggatcaccaagtactcgc <<<<<<<<<<<<<<<< 540 caaagcaccatgtgctgtcacccaacgtgtcaggtcacgtcagagccctggcggccgggg <<<<< 600 acggtgctggacaggctgagggccacctcacacaccctggcgctgagacagcccag KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 61 20 61.14 60.00 4.00 2.00 11.00 cgtgtcctcagcttctccag RIGHT PRIMER 544 21 59.25 47.62 6.00 2.00 11.00 ctttggcgagtacttggtgat PRODUCT SIZE: 484, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 2 LEFT PRIMER 61 20 61.14 60.00 4.00 2.00 11.00 cgtgtcctcagcttctccag RIGHT PRIMER 539 21 59.23 52.38 6.00 1.00 11.00 gcgagtacttggtgatcctgt PRODUCT SIZE: 479, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 3 LEFT PRIMER 61 19 60.12 57.89 4.00 0.00 11.00 cgtgtcctcagcttctcca RIGHT PRIMER 530 22 59.89 45.45 4.00 1.00 12.00 tggtgatcctgtttgttactgg PRODUCT SIZE: 470, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 4 LEFT PRIMER 175 19 60.51 63.16 5.00 2.00 12.00 gtgggtctcctgggacaag RIGHT PRIMER 544 21 59.25 47.62 6.00 2.00 11.00 ctttggcgagtacttggtgat PRODUCT SIZE: 370, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 Statistics con too in in no tm tm high high high high sid many tar excl bad GC too too any 3' lib poly end ered Ns get reg GC% clamp low high compl compl sim X stab ok Left 1594 0 0 0 1191 0 0 0 2 98 25 0 58 220 Right 1667 0 0 0 1097 0 0 0 4 180 38 0 27 321 Pair Stats: considered 56, unacceptable product size 46, high end compl 2, ok 8 primer3 release 1.0