PRIMER PICKING RESULTS FOR NM_006162_6 Using mispriming library /data/utswmc/elxr/ELXR-main/repbase/humrep_and_simple.fa Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 142 21 59.97 57.14 4.00 2.00 12.00 cccctagaagtagtggcatcc RIGHT PRIMER 628 19 60.61 52.63 5.00 0.00 11.00 agctgtcggctcacacaaa SEQUENCE SIZE: 741 INCLUDED REGION SIZE: 741 PRODUCT SIZE: 487, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 269,201 0 gcttcccctcacgctgcgctgacctttgcgtatttgtgcgatgactgtgatttgagtgaa 60 attggctgagtcttaggaagatattttgatgacttcttcagaacgaggtggagaccatca 120 ggatgaagtgtgcacggcttctcccctagaagtagtggcatccgcaggggtcggcccgca >>>>>>>>>>>>>>>>>>>>> 180 ggtcgtggcggtgcttgctctgggtgggtcggctcacgtgtgacctgctgggtgccacac 240 gtgtgccccaggccgcccactcaggacaggccctcactgcccctctccttctgatgcagC ******************************* 300 CCAGCGCTCAGCTCAGGAGCTGCCTCTGGTGGAGAAGCAGAGCACGGACAGCTATCCGGT ************************************************************ 360 CGTGGGCGGGAAGAAGATGGTCCTGTCTGGCCACAACTTCCTGCAGGACTCCAAGGTCAT ************************************************************ 420 TTTCGTGGAGAAAGCCCCAGgtatgctcttcaccaggggccatctgcggcctgggcttcg ************************************************** 480 gcgctggtgggaaccggttcccagtcggtggagcaggacccacctgtgcacggtcaccgg 540 gacggggcttatggggtgtggcacggagcagaggctctgcgtggcccgtgtctgcatccg 600 gctgtgggtttttgtgtgagccgacagctgtgctgctgaagggaagaggtggatgtttat <<<<<<<<<<<<<<<<<<< 660 ttctggggccgccggggccactgcactcacatcgctgctgatactcagggagcccacggg 720 ctggggacgcagaggagggca KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 142 21 59.97 57.14 4.00 2.00 12.00 cccctagaagtagtggcatcc RIGHT PRIMER 624 19 59.13 52.63 2.00 0.00 12.00 gtcggctcacacaaaaacc PRODUCT SIZE: 483, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00 2 LEFT PRIMER 142 21 59.97 57.14 4.00 2.00 12.00 cccctagaagtagtggcatcc RIGHT PRIMER 627 19 61.02 52.63 4.00 0.00 12.00 gctgtcggctcacacaaaa PRODUCT SIZE: 486, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00 3 LEFT PRIMER 134 21 59.51 57.14 5.00 3.00 12.00 cggcttctcccctagaagtag RIGHT PRIMER 628 19 60.61 52.63 5.00 0.00 11.00 agctgtcggctcacacaaa PRODUCT SIZE: 495, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00 4 LEFT PRIMER 142 21 59.97 57.14 4.00 2.00 12.00 cccctagaagtagtggcatcc RIGHT PRIMER 563 18 59.93 61.11 5.00 2.00 11.00 gtgccacaccccataagc PRODUCT SIZE: 422, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00 Statistics con too in in no tm tm high high high high sid many tar excl bad GC too too any 3' lib poly end ered Ns get reg GC% clamp low high compl compl sim X stab ok Left 1440 0 0 0 760 0 0 0 1 271 62 0 45 301 Right 1489 0 0 0 1014 0 0 0 8 163 42 0 46 216 Pair Stats: considered 2029, unacceptable product size 1951, tm diff too large 64, ok 14 primer3 release 1.0