PRIMER PICKING RESULTS FOR NM_006162_5 Using mispriming library /data/utswmc/elxr/ELXR-main/repbase/humrep_and_simple.fa Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 139 21 59.35 42.86 6.00 0.00 12.00 tggcttcaaggtcttgttttc RIGHT PRIMER 572 21 60.16 52.38 4.00 0.00 11.00 gtcccaagtctacacgaacga SEQUENCE SIZE: 773 INCLUDED REGION SIZE: 773 PRODUCT SIZE: 434, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 269,233 0 gtggagggtggaggctggaggctggaggaaggtgggaaggggaaggtggaagtggctttt 60 ctcggggctgcctctgcttgccccctcgcctgcgccaatgtcagattctctcctgcacta 120 ggcaagggcctcacgtcgctggcttcaaggtcttgttttccttggggaaggggagagtgg >>>>>>>>>>>>>>>>>>>>> 180 ccagctgcctggcacgagctcgtggggctgggatgagggccagagggtctggggggccag 240 gcctttacgctcccgcgtcagccattgaaagaaaagctgtgggcttttgttttgggcagC ******************************* 300 ATTGACTGTGCCGGAATCCTGAAACTCAGAAACTCCGACATTGAACTTCGGAAAGGAGAG ************************************************************ 360 ACGGACATCGGGAGGAAGAACACACGGGTACGGCTGGTGTTCCGCGTTCACGTCCCGCAA ************************************************************ 420 CCCAGCGGCCGCACGCTGTCCCTGCAGGTGGCCTCCAACCCCATCGAATGCTgtaagtgg ************************************************************ 480 acgtccacgcgcccacaggggaggaaaccgtcccgtcacatgcgcttcactgtctcaccc ********************** 540 gctctcagcttttcgttcgtgtagacttgggactgaacggttcccaccaaacccaccaca <<<<<<<<<<<<<<<<<<<<< 600 gtgtgtggccacgaggggtctgagttgatcatggctgagttattgcctgtggcctcccgg 660 gggcccctggcgctggctcctctgatggctgagcatggccagggaaagcggtgtgggccg 720 gggcttcccctcacgctgcgctgacctttgcgtatttgtgcgatgactgtgat KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 137 21 60.79 47.62 6.00 0.00 12.00 gctggcttcaaggtcttgttt RIGHT PRIMER 570 21 60.15 47.62 4.00 0.00 11.00 cccaagtctacacgaacgaaa PRODUCT SIZE: 434, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00 2 LEFT PRIMER 137 21 60.79 47.62 6.00 0.00 12.00 gctggcttcaaggtcttgttt RIGHT PRIMER 572 21 60.16 52.38 4.00 0.00 11.00 gtcccaagtctacacgaacga PRODUCT SIZE: 436, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 3 LEFT PRIMER 139 21 59.35 42.86 6.00 0.00 12.00 tggcttcaaggtcttgttttc RIGHT PRIMER 561 21 59.67 47.62 4.00 1.00 12.00 acacgaacgaaaagctgagag PRODUCT SIZE: 423, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 4 LEFT PRIMER 137 21 60.79 47.62 6.00 0.00 12.00 gctggcttcaaggtcttgttt RIGHT PRIMER 561 21 59.67 47.62 4.00 1.00 12.00 acacgaacgaaaagctgagag PRODUCT SIZE: 425, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 Statistics con too in in no tm tm high high high high sid many tar excl bad GC too too any 3' lib poly end ered Ns get reg GC% clamp low high compl compl sim X stab ok Left 1472 0 0 0 989 0 0 0 0 118 51 1 50 263 Right 1274 0 0 0 564 0 0 0 0 161 70 0 70 409 Pair Stats: considered 29, unacceptable product size 17, high end compl 2, ok 10 primer3 release 1.0