PRIMER PICKING RESULTS FOR NM_006162_4 Using mispriming library /data/utswmc/elxr/ELXR-main/repbase/humrep_and_simple.fa Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 228 18 59.98 61.11 4.00 1.00 12.00 ctctgcgttccggtgact RIGHT PRIMER 699 21 60.25 52.38 6.00 2.00 11.00 acagggctgaaaccctagaga SEQUENCE SIZE: 803 INCLUDED REGION SIZE: 803 PRODUCT SIZE: 472, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 269,263 0 ctgcacacgccccctgccagcttcactgtggcctcagaatgggcacacacagcaagtgtg 60 gatgcatacgtgcaaggcccgtttcaaagcctaaaactcagtgcccaggtccattgggat 120 aacaaggcattttctaatctgatttacaaagacaccagtatgacatggcagagaaataaa 180 aagggggcagggcagggggacacaggcctcgaacccgccgcaggttttctctgcgttccg >>>>>>>>>>>> 240 gtgactcccggcggtctgtgctctgggtgctgagcaggtgttttctgttctctcgccagC >>>>>> ******************************* 300 TGCATGGCTACTTGGAGAATGAGCCGCTGATGCTGCAGCTTTTCATTGGGACGGCGGACG ************************************************************ 360 ACCGCCTGCTGCGCCCGCACGCCTTCTACCAGGTGCACCGCATCACAGGGAAGACCGTGT ************************************************************ 420 CCACCACCAGCCACGAGGCCATCCTCTCCAACACCAAAGTCCTGGAGATCCCACTCCTGC ************************************************************ 480 CGGAGAACAGCATGCGAGCCGTgtaagccgcgggggacctccggcctctggcagggggcg **************************************************** 540 gtaggagggggcgtgcctccctcccagtcccggggggtctggccagccccccgcacttcc 600 aggctgcgtggccaggacacttttggtttaacagccaataagtaaacttcactcttgctt 660 ttgacaatggatttgcttctctctagggtttcagccctgtgtatattgccacttaaaatg <<<<<<<<<<<<<<<<<<<<< 720 tcagatatgcagctataaatacctccctctgcatttagcttctaaacaggacccctattt 780 ctttccgaatacagcagtgtaat KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 228 18 59.98 61.11 4.00 1.00 12.00 ctctgcgttccggtgact RIGHT PRIMER 698 21 60.38 57.14 6.00 2.00 12.00 cagggctgaaaccctagagag PRODUCT SIZE: 471, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 2 LEFT PRIMER 228 18 59.98 61.11 4.00 1.00 12.00 ctctgcgttccggtgact RIGHT PRIMER 697 21 59.47 52.38 6.00 0.00 12.00 agggctgaaaccctagagaga PRODUCT SIZE: 470, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00 3 LEFT PRIMER 228 18 59.98 61.11 4.00 1.00 12.00 ctctgcgttccggtgact RIGHT PRIMER 676 21 59.22 38.10 5.00 2.00 11.00 agcaaatccattgtcaaaagc PRODUCT SIZE: 449, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 4 LEFT PRIMER 228 18 59.98 61.11 4.00 1.00 12.00 ctctgcgttccggtgact RIGHT PRIMER 675 21 61.00 38.10 5.00 1.00 11.00 gcaaatccattgtcaaaagca PRODUCT SIZE: 448, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 Statistics con too in in no tm tm high high high high sid many tar excl bad GC too too any 3' lib poly end ered Ns get reg GC% clamp low high compl compl sim X stab ok Left 1334 0 0 0 546 0 0 0 11 298 47 0 59 373 Right 1598 0 0 0 639 0 0 0 1 289 47 0 38 584 Pair Stats: considered 1401, unacceptable product size 1351, tm diff too large 34, high end compl 7, ok 9 primer3 release 1.0