PRIMER PICKING RESULTS FOR NM_006162_3 Using mispriming library /data/utswmc/elxr/ELXR-main/repbase/humrep_and_simple.fa Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 146 21 59.93 47.62 4.00 2.00 12.00 actgcattgacacaggcttct RIGHT PRIMER 586 21 59.23 52.38 2.00 2.00 12.00 ggactaaccacgacagagcat SEQUENCE SIZE: 760 INCLUDED REGION SIZE: 760 PRODUCT SIZE: 441, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00 TARGETS (start, len)*: 269,220 0 gagttctaaaacctgtcttactggagggttaagtaactgatagaagcctgtcatgaagga 60 agagaagcgtgttaaggttgtgcttggaaaatgtttccatcttagagaactggccgggcc 120 gtggccacaggtcacgatgagtaagcactgcattgacacaggcttctccatctccatgtc >>>>>>>>>>>>>>>>>>>>> 180 aggacgtgcactcgccgatgaagtgtccacccaagatggcgacgggtgagcacgggcacg 240 tgtggcccgggcgaggtctgtgtggtgctgaacgcctcctctgctctgttcccttccagC ******************************* 300 CCGACCCTGCCCGCCCTGGACTGGCAGCTGCCGTCCCACTCAGGCCCGTATGAGCTTCGG ************************************************************ 360 ATTGAGGTGCAGCCCAAGTCCCACCACCGAGCCCACTACGAGACGGAGGGCAGCCGGGGG ************************************************************ 420 GCCGTGAAGGCGTCGGCCGGAGGACACCCCATCGTGCAGgtaggcactgcggccagactc ************************************************************ 480 gcacgtcacttggtgcttttgtggtcaaaaagtaactttggagccacagctaactgtgct ********* 540 tagactctcccagccaggccagctgaatgctctgtcgtggttagtcccgggcggctgctc <<<<<<<<<<<<<<<<<<<<< 600 accgcctctgagctggaatgggagcatgccacagctcctcctgcttccacacgtgcaggc 660 cctctctcgttgttttcagatttgcccgaagcagctctcgtatccgtggatgctgcctgt 720 ggtgtggatgttttgggggttgattctcccctctctaaag KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 141 21 59.92 47.62 6.00 1.00 11.00 taagcactgcattgacacagg RIGHT PRIMER 586 21 59.23 52.38 2.00 2.00 12.00 ggactaaccacgacagagcat PRODUCT SIZE: 446, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 2 LEFT PRIMER 161 21 59.82 52.38 4.00 2.00 12.00 gcttctccatctccatgtcag RIGHT PRIMER 586 21 59.23 52.38 2.00 2.00 12.00 ggactaaccacgacagagcat PRODUCT SIZE: 426, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00 3 LEFT PRIMER 162 21 59.66 52.38 4.00 3.00 12.00 cttctccatctccatgtcagg RIGHT PRIMER 586 21 59.23 52.38 2.00 2.00 12.00 ggactaaccacgacagagcat PRODUCT SIZE: 425, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 4 LEFT PRIMER 146 21 59.93 47.62 4.00 2.00 12.00 actgcattgacacaggcttct RIGHT PRIMER 508 20 60.13 40.00 4.00 0.00 11.00 tttgaccacaaaagcaccaa PRODUCT SIZE: 363, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 Statistics con too in in no tm tm high high high high sid many tar excl bad GC too too any 3' lib poly end ered Ns get reg GC% clamp low high compl compl sim X stab ok Left 1463 0 0 0 469 0 0 0 8 350 47 0 45 544 Right 1262 0 0 0 307 0 0 0 23 329 66 0 61 476 Pair Stats: considered 1098, unacceptable product size 1085, tm diff too large 4, ok 9 primer3 release 1.0