PRIMER PICKING RESULTS FOR NM_000438_3 Using mispriming library /data/utswmc/elxr/ELXR-main/repbase/humrep_and_simple.fa Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 47 21 59.81 42.86 4.00 1.00 11.00 tgaatttatcccggttgtctg RIGHT PRIMER 521 21 59.67 42.86 4.00 2.00 12.00 tcgataattggggtgattacg SEQUENCE SIZE: 730 INCLUDED REGION SIZE: 730 PRODUCT SIZE: 475, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 TARGETS (start, len)*: 269,190 0 agaaagtataaccccaccctacccccatcaagatgttataattcttctgaatttatcccg >>>>>>>>>>>>> 60 gttgtctggggcttgtctggggaagttagaggcttcccaggctgctccgggatgggatgg >>>>>>>> 120 gatgtgttctggtctgcccctttctaaagtgcctcagagagttcaggtctttttgctcac 180 aaagggggaccgtcccagaggattcagcgaggagcatcccgacaggcccagaggcggtgg 240 ggccgccgccacctggcccagggtaccgggtaccaacgcctgcccgcctgttctcttaaa ******************************* 300 gCAGGTGACAACGCCTGACGTGGAGAAGAAAATTGAGGAATACAAAAGAGAGAACCCGGG ************************************************************ 360 CATGTTCAGCTGGGAAATCCGAGACAAATTACTCAAGGACGCGGTCTGTGATCGAAACAC ************************************************************ 420 CGTGCCGTCAGgtactaggcccattaacctctccccgaggcgcgacagtcagtcgctatt *************************************** 480 accccggcacgtcgacccagacgtaatcaccccaattatcgatctcagctattgggaggt <<<<<<<<<<<<<<<<<<<<< 540 ggcctctcccgaggccctgcaatatttgccaacccggtgttcttggcactttatggaggg 600 aggggtgagctgcttgtcctggggacggagggtggcgagaccaccgctgaggacgcccca 660 aagaagatttctgtcgaggaggcagggcaggaaacgtgaggcggaggcagagcgtttatt 720 aatattgatg KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 47 21 59.81 42.86 4.00 1.00 11.00 tgaatttatcccggttgtctg RIGHT PRIMER 541 21 60.47 52.38 4.00 3.00 12.00 ccacctcccaatagctgagat PRODUCT SIZE: 495, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00 2 LEFT PRIMER 115 21 60.38 52.38 2.00 1.00 11.00 gatgggatgtgttctggtctg RIGHT PRIMER 521 21 59.67 42.86 4.00 2.00 12.00 tcgataattggggtgattacg PRODUCT SIZE: 407, PAIR ANY COMPL: 2.00, PAIR 3' COMPL: 0.00 3 LEFT PRIMER 47 21 59.81 42.86 4.00 1.00 11.00 tgaatttatcccggttgtctg RIGHT PRIMER 539 21 60.60 52.38 4.00 2.00 10.00 acctcccaatagctgagatcg PRODUCT SIZE: 493, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00 4 LEFT PRIMER 115 21 60.38 52.38 2.00 1.00 11.00 gatgggatgtgttctggtctg RIGHT PRIMER 541 21 60.47 52.38 4.00 3.00 12.00 ccacctcccaatagctgagat PRODUCT SIZE: 427, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00 Statistics con too in in no tm tm high high high high sid many tar excl bad GC too too any 3' lib poly end ered Ns get reg GC% clamp low high compl compl sim X stab ok Left 1298 0 0 0 609 0 0 0 9 205 55 0 70 350 Right 1334 0 0 0 550 0 0 0 0 249 45 0 84 406 Pair Stats: considered 8, ok 8 primer3 release 1.0