PRIMER PICKING RESULTS FOR NM_000438_2 Using mispriming library /data/utswmc/elxr/ELXR-main/repbase/humrep_and_simple.fa Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 229 21 59.69 42.86 3.00 0.00 12.00 gtgctgctgttctttgctttt RIGHT PRIMER 668 21 60.16 47.62 2.00 0.00 12.00 ttacgcaccttcacaaacctc SEQUENCE SIZE: 836 INCLUDED REGION SIZE: 836 PRODUCT SIZE: 440, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 269,296 0 ctgcagggtctcctaagttgccactccccgggcccgaaggggaagacgctttctctgggg 60 agaattattttgccccatttgctgtctttacacaagattacatgcgctatattgctgaat 120 aatttgaacaaatgtgattgaaaggaggtctgggtctgggagtccgatgtcgagcagttt 180 cagcgtcggtgctgtaacatgaggataggacagaagactgcgaaattacgtgctgctgtt >>>>>>>>>>> 240 ctttgctttttattttcctccagtgacttttcccttgcttctctttttcaccttcccaca >>>>>>>>>> ******************************* 300 gTGTCCACTCCCCTCGGCCAGGGCCGCGTCAACCAGCTCGGCGGTGTTTTTATCAACGGC ************************************************************ 360 AGGCCGCTGCCCAACCACATCCGCCACAAGATCGTGGAGATGGCCCACCACGGCATCCGG ************************************************************ 420 CCCTGCGTCATCTCGCGCCAGCTGCGCGTGTCCCACGGCTGCGTCTCCAAGATCCTGTGC ************************************************************ 480 AGGTACCAGGAGACTGGCTCCATACGTCCTGGTGCCATCGGCGGCAGCAAGCCCAAGgtg ************************************************************ 540 agcgggcgggccttgccctcctggctcccagactgtggtcccctgtgttggggaagaccg ************************* 600 ggcggggggtaacggctgacatctggggctctccaatgagttatttctgaggtttgtgaa <<<<<<<<<<<< 660 ggtgcgtaaaggcgcgcgtgtgtgtgtgtacgtgtgcacgcgccctggacacttcttgat <<<<<<<<< 720 gaacacttagggaaagctcttgcctttttgcaaaggaaaaataacatctgttttttaaag 780 cccataagccttgcaatcctcatcaaattattttaatggataaacacctaaagcag KEYS (in order of precedence): ****** target >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 229 21 59.69 42.86 3.00 0.00 12.00 gtgctgctgttctttgctttt RIGHT PRIMER 669 21 59.66 42.86 3.00 0.00 11.00 tttacgcaccttcacaaacct PRODUCT SIZE: 441, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 2 LEFT PRIMER 229 21 59.69 42.86 3.00 0.00 12.00 gtgctgctgttctttgctttt RIGHT PRIMER 670 21 59.66 47.62 3.00 0.00 10.00 ctttacgcaccttcacaaacc PRODUCT SIZE: 442, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 3 LEFT PRIMER 229 21 59.69 42.86 3.00 0.00 12.00 gtgctgctgttctttgctttt RIGHT PRIMER 671 21 59.66 47.62 3.00 0.00 11.00 cctttacgcaccttcacaaac PRODUCT SIZE: 443, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00 4 LEFT PRIMER 185 21 60.53 47.62 5.00 2.00 12.00 tcggtgctgtaacatgaggat RIGHT PRIMER 664 21 60.28 47.62 3.00 1.00 12.00 gcaccttcacaaacctcagaa PRODUCT SIZE: 480, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 Statistics con too in in no tm tm high high high high sid many tar excl bad GC too too any 3' lib poly end ered Ns get reg GC% clamp low high compl compl sim X stab ok Left 1322 0 0 0 360 0 0 0 6 356 86 0 49 465 Right 1326 0 0 0 601 0 0 0 0 279 63 13 50 320 Pair Stats: considered 33, unacceptable product size 18, high end compl 3, ok 12 primer3 release 1.0