PRIMER PICKING RESULTS FOR NM_000438_1p2 Using mispriming library /data/utswmc/elxr/ELXR-main/repbase/humrep_and_simple.fa Using 0-based sequence positions OLIGO start len tm gc% any 3' rep seq LEFT PRIMER 622 21 59.56 42.86 4.00 2.00 11.00 TTCGCCTTCACCTGGATATAA RIGHT PRIMER 1015 21 59.99 42.86 3.00 0.00 12.00 ttccaaaacaacagggacaag SEQUENCE SIZE: 1051 INCLUDED REGION SIZE: 1051 PRODUCT SIZE: 394, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 TARGETS (start, len)*: 669,112 EXCLUDED REGIONS (start, len)*: 0,369 1051,0 0 aggaacttctccgccctcagcaactgccatctccctggggctgtctctctcagtttccac XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60 cgggccaaccttctctcctgggcaaggggcgcagcgcgggtccccctcggggccagcaga XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120 ggcctcggcaccaccagagatgggaagagaaagtggtcgctgttgcccaatcagcgcgtg XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180 tctccgccacccgggacggtctacccgtcggccaatcgcagctcagggctcctgaccaag XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240 ctttgggtaaaagaactaataaatgctcccgagcccggatccccgcactcggtgtcacca XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 300 cAGGAGGAGACTCAGGCAGGCCGCGCTCCAGCCTCACCAGGCTCCCCGGCTCGCCGTGGC XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 360 TCTCTGAGCCCCCTTTTCAGGGACCCCAGTCGCTGGAACATTTGCCCAGACTCGTACCAA XXXXXXXXX 420 ACTTTTCCGCCCTGGGCTCGGGATCCTGGACTCCGGGGCCTCCCCGTCCTCCCCTTTCCC 480 GGGTTCCAGCTCCGGCCTCTGGACTAGGAACCGACAGCCCCCCTCCCCGCGTCCCTCCCT 540 CTCTCTCCAGCCGTTTTGGGGAGGGGCTCTCCACGCTCCGGATAGTTCCCGAGGGTCATC 600 CGCGCCGCACTCGCCTTTCCGTTTCGCCTTCACCTGGATATAATTTCCGAGCGAAGCTGC >>>>>>>>>>>>>>>>>>>>> 660 CCCCAGGATGACCACGCTGGCCGGCGCTGTGCCCAGGATGATGCGGCCGGGCCCGGGGCA *************************************************** 720 GAACTACCCGCGTAGCGGGTTCCCGCTGGAAGgtaagggagggcctcagcgcgccgcctg ************************************************************ 780 gatcccagggcctgggaccggctgcctcaccccatccccaggctccgcaggctcctttgg * 840 tgcttccaggaagcccattccctgggcaccccacaccccaagaagcaccagtcgggggcg 900 aggacctactcgatttcctttctgcaaatggagcgcgctgctctctgcaaatcctggcgg 960 agctgggcggtcaggcctgcggcgagccggggagacttgtccctgttgttttggaaatgc <<<<<<<<<<<<<<<<<<<<< 1020 cgcggtggagaaggaggtgaaccggcttctg KEYS (in order of precedence): XXXXXX excluded region ****** target >>>>>> left primer <<<<<< right primer ADDITIONAL OLIGOS start len tm gc% any 3' rep seq 1 LEFT PRIMER 638 21 59.49 42.86 4.00 2.00 11.00 TATAATTTCCGAGCGAAGCTG RIGHT PRIMER 1015 21 59.99 42.86 3.00 0.00 12.00 ttccaaaacaacagggacaag PRODUCT SIZE: 378, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00 2 LEFT PRIMER 622 21 59.56 42.86 4.00 2.00 11.00 TTCGCCTTCACCTGGATATAA RIGHT PRIMER 1016 21 60.36 38.10 4.00 0.00 12.00 tttccaaaacaacagggacaa PRODUCT SIZE: 395, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00 3 LEFT PRIMER 638 21 59.49 42.86 4.00 2.00 11.00 TATAATTTCCGAGCGAAGCTG RIGHT PRIMER 1016 21 60.36 38.10 4.00 0.00 12.00 tttccaaaacaacagggacaa PRODUCT SIZE: 379, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00 4 LEFT PRIMER 620 20 60.07 50.00 3.00 2.00 12.00 GTTTCGCCTTCACCTGGATA RIGHT PRIMER 1015 21 59.99 42.86 3.00 0.00 12.00 ttccaaaacaacagggacaag PRODUCT SIZE: 396, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00 Statistics con too in in no tm tm high high high high sid many tar excl bad GC too too any 3' lib poly end ered Ns get reg GC% clamp low high compl compl sim X stab ok Left 2042 0 0 370 1059 0 0 0 0 144 28 1 84 356 Right 1481 0 0 0 883 0 0 0 14 221 31 0 65 267 Pair Stats: considered 16, unacceptable product size 8, high end compl 1, ok 7 primer3 release 1.0